The purpose of this experiment is to examine if G. lucidum extracts could have an effect on leukocyte migration and to select the best chemotactic factor for leukocyte migration.
When chemotactic factors such as TC-199 medium, filtrate of E. coli culture, N-fMLP, and G. lucidum extracts were used, N-fMLP, G. lucidum extracts and filtrate of E. coli were positive effect, but TC-199 medium was less than others.
The various concentrations of the chemotactic factors such as G. lucidum extracts(1.00%, 0.10% and 0.01%) were stimulated for the leukocytes migration in the modified Boyden chambers, however, the lowest concentration such as 0.01% was more effective than others for the chemoattractant of the leukocyte migration
When the leukocyte was treated with G. lucidum extracts(1.00%, 0.10%, and 0.01%) at room temperature for 120 minutes, the chemotactic activities had a good effect at 0. 01%. Therefore, leukocyte was affected by G. lucidum extracts.
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